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| Aminot, Alain; Kerouel, Roger; Birot, Dominique. |
| A version of the orthophthaldialdehyde-fluorescence ammonium determination for flow injection analysis (FIA) is presented here, with a view to its use for in situ, low-power consumption systems, Thus, the reaction temperature was limited to 30 degreesC and FIA was used in stop-Bow mode (3 min stop). The calibration is linear up to 50 mu mol l(-1), but concentrations up to 100 mu mol l(-1) can be measured. Repeatability is around 1% in the range of 0.5-4 mu mol l(-1) and the detection limit is about 0.03 mu mol l(-1) Over the salinity range of 5-35 (seawater practical salinity scale) the salt effect is almost negligible (within +/- 2%); and below salinity of 5 it increases to a maximum of -9% in fresh water compared to seawater. Hydrogen carbonate,... |
| Tipo: Text |
Palavras-chave: In situ; Fluorometry; Flow injection analysis; Determination; Ammonium. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-1722.pdf |
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| Coverly, Stephen; Kerouel, Roger; Aminot, Alain. |
| Modern instruments together with the use of standard reference materials have improved the accuracy and long-term reproducibility of the analysis of nutrients (ammonium, nitrate, nitrite, phosphate and silicate) in sea water using segmented-flow analysis, so that errors arising from matrix effects become more significant. Colorimetric detectors with bubble-through flowcells have become widely used for seawater analysis in recent years and their associated matrix effects are described. A re-examination of all categories of matrix effects, whose main origin is salinity, was thus undertaken to assess how much they are liable to alter the data. Interferences were classified into four types, each of which was examined in order to show its influence on the... |
| Tipo: Text |
Palavras-chave: Nutrient; Determination; Seawater; Estuarine water; Segmented-flow analysis; Matrix effects. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00057/16843/14530.pdf |
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| Nozawa,Carlos M; Kerntopf,Gustavo Franthesco; Czernisz,Érika da Silva; Albuquerque,Daniele; Romanin,Priscila; Freitas,José Felipe Eliseu; Santos,Norma; Benati,Fabrício José; Pietruchinski,Eduardo; Linhares,Rosa Elisa Carvalho. |
| Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under five years of age are the target population. OBJECTIVE: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai - Paraná. METHODS: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. RESULT: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Determination; Hospitalized patients; Genotyping. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600002 |
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| Gras, Marie-paule; Gras, Paul. |
| Following the high mortality which killed the Portuguese oysters Crassostrea angulata cultivated on the French coasts, the massive establishment of Pacific oysters Crassostrea gigas, resisting the disease and the creation of artificial deposits for the latter was decided. In most culture areas, the mortality was estimated at more than 80 % of the stock, but, in some regions, some small populations of Crassostrea angulata subsisted and kept on reproducing. A genetic control of the spawner stocks had to be made. The first step was to know if the oysters living in the same areas were of the same species in order to foresee the next generations and try to improve them (selection). |
| Tipo: Text |
Palavras-chave: Sélection; Determination; Crassostrea gigas; Crassostea angulata; Huîtres; Génétique. |
| Ano: 1977 |
URL: http://archimer.ifremer.fr/doc/1977/acte-6579.pdf |
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| Aminot, Alain; Kerouel, Roger. |
| The paper reviews the methods described in the literature for the determination of total dissolved free primary amines (TDFPA) by fluorescence. A wide set of reaction conditions can be found, but they rely on few experiments for their validation. Among fluorogenic compounds, o-plithaldialdehyde (OPA) is more sensitive than fluorescamine and was thus examined here. However, the use of mercaptoethanol (ME) in the reaction (as ail additional derivatization compound) is able to generate unreliable results, in particular when standardization relies on glycine. We suggest replacing ME with 3-mercaptopropionic acid (MPA) which induces more stable and comparable fluorescence among amine Compounds. A systematic study was therefore undertaken to define reagent... |
| Tipo: Text |
Palavras-chave: Fluorescence; Determination; Seawater; Total dissolved free primary amines. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2274.pdf |
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